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US 10,870,669 B2Granted
Method for producing an isolated monoclonal antibody specifically binding to a glycolipid antigen of mycoplasma pneumoniae
AssigneeM Bio Technology Inc.
InventorsKazuhiro Matsuda, Yuko Shingu
Life of this patent
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Abstract
The present invention provides a novel glyceroglycolipid produced by Mycoplasma pneumoniae. The glyceroglycolipid can be used as a diagnostic marker for a disease caused by Mycoplasma pneumoniae.
Claims4
A method for producing an isolated monoclonal antibody specifically binding to a glycolipid antigen of Mycoplasma pneumoniae, comprising:
- (a) immunizing a mammal with a glycolipid antigen to produce an antibody-producing cell, wherein the glycolipid antigen is:
- (i) an isolated compound represented by the following general formula or a salt thereof:
- wherein R1═OH, R2═H and R3 is —(CH2)nCH3, wherein n is 12, 14, 16 or 18, or
- (ii) an isolated compound represented by the following general formula or a salt thereof:
- wherein R1═H and R2═OH and R3 is (CH2)nCH3, wherein n is 12, 14, 16 or 18;
- (b) obtaining an antibody-producing cell from the mammal;
- (c) fusing a myeloma cell with the antibody-producing cell to produce a hybridoma cell line;
- (d) screening the hybridoma cell line that produces a monoclonal antibody that specifically binds to the glycolipid antigen, wherein said specific binding of the monoclonal antibody to the glycolipid antigen is verifiable by an enzyme-linked immunosorbent assay using the glycolipid antigen;
- (e) culturing the screened hybridoma cell line;
- and
- (f) isolating the monoclonal antibody produced by the hybridoma cell line.
8 more elements
Dependencies are inferred from the claim text.