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US 10,870,111 B2Granted
Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
AssigneeThe University of North Carolina at Chapel Hill
InventorsJohn Michael Ramsey, William Hampton Henley
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Abstract
A fluidic device includes a plurality of reaction wells, typically in a dense array, with at least one bead retention segment in fluid communication with and spatially separated from at least one signal detection segment. A respective bead retention segment can be configured to hold a single bead, which can have a reagent attached thereto.
Claims20
A fluidic device comprising:
- a plurality of reaction wells that are in fluid isolation from each other and sealed from one another by a sealing agent, and characterized in that each reaction well has at least one bead retention segment and at least one spatially separated signal detection segment that is configured as at least one elongate channel in fluid communication with the at least one bead retention segment, wherein the at least one bead retention segment has a depth direction, wherein the at least one elongate channel extends away from the at least one bead retention segment in a length direction that is perpendicular to the depth direction, and wherein the at least one elongate channel has a length in the length direction that is greater than a width of the at least one bead retention segment;
- and
- a plurality of magnetic beads, wherein one magnetic bead of the plurality of magnetic beads is physically retained in each bead retention segment of the plurality of reaction wells.
A fluidic device comprising:
- a plurality of reaction wells characterized in that each reaction well has at least one bead retention segment and at least one spatially separated signal detection segment that is configured as at least one elongate channel in fluid communication with the at least one bead retention segment, wherein the at least one bead retention segment has a depth direction, wherein the at least one elongate channel extends away from the at least one bead retention segment in a length direction that is perpendicular to the depth direction, and wherein the at least one elongate channel has a length in the length direction that is greater than a width of the at least one bead retention segment,
- wherein the device is a microfluidic chip and the plurality of reaction wells are provided as an array of reaction wells, wherein the at least one bead retention segment and the at least one signal detection segment reside in a first planar substrate with the first planar substrate defining an open upper surface and sidewalls of the at least one bead retention segment and the at least one signal detection segment, and wherein the device further comprises a second planar substrate that couples to the first planar substrate and defines a closed outer surface with at least one fluid port.
A fluidic device comprising:
- a plurality of reaction wells characterized in that each reaction well has at least one bead retention segment and at least one spatially separated signal detection segment that is configured as at least one elongate channel in fluid communication with the at least one bead retention segment, wherein the at least one bead retention segment has a depth direction, wherein the at least one elongate channel extends away from the at least one bead retention segment in a length direction that is perpendicular to the depth direction, and wherein the at least one elongate channel has a length in the length direction that is greater than a width of the at least one bead retention segment,
- wherein each of the plurality of reaction wells has a volumetric capacity in a range of 1 aL to 1 μL, and wherein the device further comprises a sealing agent that seals the plurality of reaction wells from each other.
A fluidic device comprising:
- a first substrate comprising a plurality of reaction wells characterized in that each reaction well has at least one bead retention segment and at least one spatially separated signal detection segment that is configured as at least one elongate channel in fluid communication with the at least one bead retention segment, wherein the at least one bead retention segment has a depth direction, wherein the at least one elongate channel extends away from the at least one bead retention segment in a direction that is perpendicular to the depth direction, wherein a volume capacity of each reaction well is in a range of 1 aL to 100 μL, wherein the device further comprises a spacer and a sealing agent between the first substrate and a second substrate, wherein each reaction well of the plurality of reaction wells comprises a reaction solution that does not touch the second substrate, and wherein the plurality of reaction wells are sealed from one another by the sealing agent.
A fluidic device comprising:
- a plurality of reaction wells characterized in that each reaction well has at least one bead retention segment and at least one spatially separated signal detection segment that is configured as at least one elongate channel in fluid communication with the at least one bead retention segment, wherein the at least one bead retention segment has a depth direction, wherein the at least one elongate channel extends away from the at least one bead retention segment in a length direction that is perpendicular to the depth direction, and wherein the at least one elongate channel has a length in the length direction that is greater than a width of the at least one bead retention segment,
- wherein the reaction wells are provided in a density in a range of about 6,000 wells/cm2 to about 2,000,000 wells/cm2.
Dependencies are inferred from the claim text.