Chlorella-based composition for application to plants and methods of its preparation
AssigneeHeliae Development, LLC
InventorsSandip Shinde, Stephen Ventre, Nicholas Donowitz, Michael Rohlfsen, Laura Carney
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Abstract
Methods of preparing a liquid Chlorella based composition comprising pasteurization and stabilization of a low concentration of mixotrophic or non-mixotrophic Chlorella whole cells are disclosed. The liquid composition can be used to enhance the emergence and growth of plants in low concentration and low frequency soil, foliar, seed soak, capillary action, and hydroponic applications.
Claims53
A method of preparing a whole cell Chlorella liquid plant growth treatment composition that enhances a characteristic of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells, comprising:
- heating a culture comprising the whole Chlorella cells in a liquid culture medium at a temperature in the range of 50-90° C.;
- cooling the heated composition, wherein the heating and cooling steps pasteurize the whole Chlorella cells in the composition, making them suitable in the enhancement of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells;
- and
- adjusting the composition to a pH in the range of 3-5.
A method of preparing a whole cell Chlorella liquid plant growth treatment composition that enhances a characteristic of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells, comprising:
- heating a culture comprising the whole Chlorella cells in a liquid culture medium at a temperature in the range of 50-90° C.;
- and
- cooling the heated composition, wherein the heating and cooling steps pasteurize the whole Chlorella cells in the composition, making them suitable in the enhancement of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells,
- wherein the composition comprises phytohormones selected from:
- abscisic acid, abscisic acid metabolites, cytokinins, auxins, gibberellins, and any combination thereof.
A method of preparing a whole cell Chlorella liquid plant growth treatment composition that enhances a characteristic in a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells, comprising:
- heating a culture comprising the whole Chlorella cells in a liquid culture medium at a temperature in the range of 50-90° C.;
- cooling the heated composition, wherein the heating and cooling steps pasteurize the whole Chlorella cells in the composition, making them suitable in the enhancement of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells;
- and
- contacting the composition with a culture stabilizer suitable for plants, wherein the culture stabilizer is selected from:
- potassium sorbate, phosphoric acid, ascorbic acid, sodium benzoate, and any combination thereof.
Dependencies are inferred from the claim text.