HAV detection
AssigneeRoche Molecular Systems, Inc.
InventorsHermann Leying, Ning Lu, Nick Newton, Andreas Wolfelschneider, Karen Young, Dirk Zimmermann
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Abstract
Methods for detecting HAV in a biological sample are provided, comprising amplifying a target nucleic acid comprising the sequence of HAV in a reaction mixture. The reaction mixture comprises a biological sample which may contain the target nucleic acid and set of oligonucleotides. The invention also provides kits for the detection of HAV.
Claims3
A reaction mixture comprising a forward primer and a reverse primer, and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the forward primer consists of SEQ ID NO:
- 7 and the nucleic acid sequence of the reverse primer consists of SEQ ID NO:
- 11, and the nucleic acid of the probe consists of SEQ ID NO:
- 14.
A kit for detecting Hepatitis A Virus (HAV) in a biological sample comprising a polymerase, nucleotides and isolated nucleic acid sequences comprising a forward primer and a reverse primer 1 and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the forward primer consists of SEQ ID NO:
- 7 and the nucleic acid sequence of the reverse primer consists of SEQ ID NO:
- 11, and the nucleic acid of the probe consists of SEQ ID NO:
- 14.
A set of oligonucleotides for amplifying and detecting Hepatitis A Virus (HAV) in a biological sample comprising a forward and a reverse primer and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the first primer consists of SEQ ID NO:
- 7 and the nucleic acid sequence of the second primer consists of SEQ ID NOS:
- 11, and the nucleic acid of the probe consists of SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.