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AssigneesInstitut National De Recherche Pour L'agriculture, Centre National De La Recherche Scientifique, Universite De Strasbourg
InventorsNalini Rama Rao, Didier Rognan, Didier Lereclus
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Embodiments of the present disclosure relate to methods of screening and optionally designing compounds as inhibitors of Mfd (Mutation Frequency Decline), such compounds being useful for the treatment of bacterial infections. Embodiments of the methods include culturing pathogenic bacteria expressing functional Mfd with various concentrations of 1 mM or less, incubating the cultures with and without nitric oxide stress conditions, evaluating bacterial survival in each of the cultures, and calculating the concentration of the molecule required to decrease by 50% the survival rate of the bacteria (IC50) by comparing the cfu of the untreated cultures and cultures following nitric oxide stress.
An in vitro method of screening an antibacterial molecule that inhibits mutation frequency decline (Mfd peptide) activity comprising the steps of:
Dependencies are inferred from the claim text.