US 10,865,429 B2 · Microorganism having PHA synthase-coding genes and method for producing PHA using same

Abstract

An object of the invention is to improve the crystallization speed of a PHA copolymer which is to be slowly crystallized, and improve the melt workability and productivity. A microorganism is used which has genes encoding two or more different PHA synthases derived from the genus Aeromonas. The genes encoding the PHA synthases derived from the genus Aeromonas preferably include genes encoding at least two PHA synthases which are capable of synthesizing a copolymer PHA including, as monomer unit species, 3-hydroxybutyric acid and 3-hydroxyhexanoic acid, and which are different in substrate specificity toward 3-hydroxyhexanoic acid from each other. When this microorganism is cultured, a PHA mixture can be produced which includes three or more PHA species different in melting point from each other.

Claims12

Dependent claims

A microorganism, comprising genes encoding at least two PHA synthases which are different in substrate specificity toward 3-hydroxyhexanoic acid from each other and are obtained from the genus Aeromonas,

wherein the microorganism is a transformant of a host, and wherein the host is a microorganism belonging to the genus Cupriavidus, and
wherein one of the at least two PHA synthases which has a higher substrate specificity toward 3-hydroxyhexanoic acid has an amino acid sequence in which 149th asparagine in the amino acid sequence of SEQ ID NO:
1 is substituted with serine, and/or 171th aspartic acid in the amino acid sequence of SEQ ID NO:
1 is substituted with glycine.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/763,323Filed 15 Sep 2016

Priority

JP 2015-19026928 Sep 2015

Published

US 2018/0305722 A125 Oct 2018

Granted

15 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12P 7/625
Carboxylic acid esters: polyesters of hydroxy carboxylic acids
C08G 63/06
Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds: derived from hydroxycarboxylic acids
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups

Microorganism having PHA synthase-coding genes and method for producing PHA using same

Life of this patent

Microorganism having PHA synthase-coding genes and method for producing PHA using same

Life of this patent

Abstract

Claims12