Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes

A plant plastid comprising a plastid genome transformed with a heterologous expression cassette consisting of, as operably linked components in the 5′ to the 3′ direction of translation, a flanking sequence having homology to a trnl region in the plastid genome, a Prrn promoter operative in said plastid, an aadA selectable marker sequence or an antibiotic free selectable marker sequence, a psbA 5′ untranslated region sequence, a cholera toxin B subunit(CTB)-Exendin fusion gene sequence, a psbA 3′ untranslated region sequence, and a flanking sequence having homology to a trnA region in the plastid genome, said expression cassette being integrated into said plastid genome such that said CTB-Exendin is expressed in and present in said plastid, wherein the CTB-Exendin 4 fusion protein has a hinge region, and a furin cleavage site between the CTB and the Exendin 4, and wherein the hinge-furin cleavage site-exendin portion consists of SEQ ID NO:

• 7, said plastid being suitable for oral administration of said CTB-Exendin 4 to a subject in need thereof.