Use of multiple transformation enhancer sequences to improve plant transformation efficiency
AssigneeMonsanto Technology LLC
InventorsLarry A. Gilbertson, Xudong Ye
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Abstract
The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
Claims27
A method of increasing the efficiency of bacterially-mediated plant transformation, comprising the steps of:
- a) introducing at least one additional transformation enhancer sequence into a plant transformation vector;
- and
- b) transforming a plant cell with the vector by bacterially-mediated transformation, wherein the bacterium is competent for the transformation of the plant cell;
- wherein the vector comprises at least one T-DNA border sequence and a transformation enhancer sequence operably linked to the T-DNA border sequence;
- and wherein the transformation enhancer sequence comprises two or more copies of an overdrive (OD) sequence from Agrobacterium tumefaciens.
A recombinant DNA construct comprising a T-DNA border sequence, said T-DNA sequence operably linked to a transformation enhancer sequence that comprises two or more copies of an overdrive (OD) sequence selected from the group consisting of:
- a) SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:19, or SEQ ID NO:20;
- b) the sequences complementary to the full length of any of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:19, or SEQ ID NO:20;
- and
- c) combinations thereof.
Dependencies are inferred from the claim text.