Recombinant host cell engineered to overexpress helper proteins

A method of manufacturing a heterologous protein of interest in an isolated host cell comprising:

• a) providing an isolated host cell engineered to overexpress a polynucleotide encoding a helper protein having the amino acid sequence as shown in SEQ ID NO:
• 1 or a functional homologue thereof, wherein the amino acid sequence of the functional homologue has at least 95% sequence identity to the amino acid sequence as shown in SEQ ID NO:
• 1, wherein the isolated host cell comprises a heterologous polynucleotide encoding the heterologous protein of interest, wherein the heterologous protein of interest and the helper protein are different proteins, and wherein the yield of the heterologous protein of interest produced by the isolated host cell is increased as compared to a corresponding isolated host cell that does not overexpress the polynucleotide encoding the helper protein or the functional homologue;
• b) culturing the isolated host cell under suitable conditions to overexpress the helper protein or the functional homologue and express the heterologous protein of interest;
• and
• c) isolating the heterologous protein of interest from the cell culture.