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US 10,865,406 B2Granted
ALS inhibitor herbicide tolerant Beta vulgaris mutants
AssigneesBayer Intellectual Property GmbH, Kws Saat AG
InventorsRuediger Hain, Juergen Benting, Guenter Donn, Nathalie Knittel-Ottleben, Bernd Holtschulte, Andreas Loock, Clemens Springmann, Rudolf Jansen
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Abstract
The present invention relates to an ALS inhibitor herbicide tolerant Beta vulgaris plant and parts thereof comprising a mutation of an endogenous acetolactate synthase (ALS) gene, wherein the ALS gene encodes an ALS polypeptide containing an amino acid different from tryptophan at a position 569 of the ALS polypeptide.
Claims1
A method for manufacturing an ALS inhibitor herbicide tolerant Beta vulgaris plant or parts thereof, comprising:
- (a) exposing calli from B. vulgaris, to about 10−7 M-10−9 M of foramsulfuron;
- (b) selecting callus tissue from said calli, wherein said callus tissue can grow in the presence of up to 3×10−6 M foramsulfuron;
- (c) regenerating shoots from said callus tissue in presence of foramsulfuron;
- (d) regenerating plantlets from said shoots;
- (e) selecting the regenerated plantlets with foramsulfuron, iodosulfuron-methyl-sodium and/or a mixture of both, wherein the plantlets comprise a mutation at a position corresponding to nucleotides 1705-1707 of the endogenous acetolactate synthase (ALS) gene shown in reference sequence SEQ ID NO:
- 1, wherein the mutated ALS gene encodes an ALS polypeptide containing leucine at position 569 of the ALS polypeptide shown in reference amino acid sequence SEQ ID NO:
- 2, and wherein the plantlets are homozygous for the mutation of the endogenous ALS gene.
4 more elements
Dependencies are inferred from the claim text.