Back to results
US 10,865,399 B2Granted
Glucoamylase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsEsben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
Life of this patent
Estimating the term…
Abstract
The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims14
A glucoamylase variant of Penicillium having glucoamylase activity, the glucoamylase variant comprising:
- a) a substitution or deletion at two or more positions corresponding to positions 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 18, 26, 31, 33, 34, 65, 72, 74, 79, 80, 103, 105, 112, 161, 172, 218, 220, 221, 245, 253, 255, 279, 325, 327, 359, 364, 370, 375, 377, 405, 445, 447, 460, 463, 465, 468, 477, 501, 502, 504, 516, 524, 526, 563, 564, 568, 571 of the polypeptide of SEQ ID NO:
- 3;
- and
- b) an amino acid sequence that has at least 75% sequence identity to the amino acid sequence of SEQ ID NO:
- 3.
2 more elements
Dependencies are inferred from the claim text.