Compositions and methods comprising a lipolytic enzyme variant
AssigneeDanisco US Inc.
InventorsThomas P. Graycar, David A. Estell, Ayrookaran J. Poulose, Sina Pricelius
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Abstract
The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.
Claims16
A cleaning composition comprising at least one surfactant and a lipolytic enzyme variant, wherein said lipolytic enzyme variant comprises an amino acid modification to a parent lipolytic enzyme, wherein the modification is at a productive position of the lipolytic enzyme variant, wherein at least one modification of the modifications tested at the productive position meet at least one of the following criteria:
- a) a position wherein the minimum performance indices (PI) relative to Thermomyces lanuginosus lipase (TLL) parent for expression, Cotton Soils 61 (CS-61) micro-swatch activity at pH 8.2, activity on p-Nitrophenyl ester substrates at pH 6 or pH 8.2, and detergent stability, Sodium linear C11-13 alkyl benzene sulfonate (LAS) stability or thermostability are greater than or equal to 0.9, and in addition have a PI for any one of these tests that is greater than or equal to 1.0;
- b) a position wherein the minimum PI relative to TLL parent for expression, CS-61 micro-swatch activity at pH 8.2, activity on p-Nitrophenyl ester substrates at pH 6 or pH 8.2, and detergent stability, LAS stability or thermostability are greater than or equal to 0.8, and in addition have a PI for any one of these tests that is greater than or equal to 1.2;
- or
- c) a position wherein the minimum PI relative to TLL parent for expression, CS-61 micro-swatch activity at pH 8.2, activity on p-Nitrophenyl ester substrates at pH 6 or pH 8.2, and detergent stability, LAS stability or thermostability are greater than or equal to 0.5, and in addition have a PI for any one of these tests that is greater than or equal to 1.5;
- wherein the productive position is selected from the group consisting of 23, 130, 154, and 187, wherein the variant has at least 93% amino acid sequence identity to SEQ ID NO:4, with the proviso that the modification at productive position 187 is not an I, with the proviso that the modification at position 23 is not a C, and wherein the amino acid positions of the lipolytic enzyme variant are numbered by correspondence with the amino acid sequence of TLL set forth in SEQ ID NO:4.
Dependencies are inferred from the claim text.