US 10,865,386 B2 · Adult stem cells derived from human skin dermis

Abstract

Provided in the present invention are adult stem cells derived from human skin dermis, and a method for isolating same. Further provided in the present invention are osteoblastic cells and adipocytes differentiated from the adult stem cells derived from human skin dermis, and a differentiation method therefor. Further provided in the present invention is a composition for osteogenesis or lipogenesis containing the stem cells, osteoblastic cells, or adipocytes. The isolation method of the present invention enables the adult stem cells derived from human skin dermis to be obtained in an easy and simple manner at a high yield rate. Genes and growth factors which are specifically expressed in the adult stem cells derived from human skin dermis isolated using the method can be separated, identified, and used later.

Claims5

Dependent claims

A method for isolating human skin dermis-derived adult stem cells, comprising

subculturing human skin dermis-derived fibroblasts;
reacting the subcultured cells with gelatin or type 4 collagen for 1-5 minutes;
separating the cells adhering to the gelatin or type 4 collagen;
confirming vimentin and nestin are expressed in the human skin dermis-derived adult stem cells;
and
confirming S100b (S100 calcium binding protein B) gene is overexpressed in the human skin dermis-derived adult stem cells as compared to the human skin dermis-derived fibroblasts,
wherein the subculturing of human skin dermis-derived fibroblasts is subculturing fibroblasts derived from dermis of adult skin 2 to 3 times, and
wherein the stem cells are stem cells of Accession No:
KCTC11995BP.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/402,060Filed 9 Jan 2017 · division

Priority

KR 10-2011-008476824 Aug 2011

Published

US 2017/0114326 A127 Apr 2017

Granted

15 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification22 codes

C12N 5/0668
Stem cells: mesenchymal stem cells from other natural sources
A61K 35/28
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 35/32
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 35/35
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…

Family

PCT/KR2012/006780Parent of this division · filed 24 Aug 2012

US 2017/0114326 A1Pre-grant publication · published 27 Apr 2017

Application14/240,330Parent of this division

US 2014/0170122 A1Published application

US 10,865,386 B2This patent

From the US filing history. Foreign family members aren’t listed.

Adult stem cells derived from human skin dermis

Life of this patent

Adult stem cells derived from human skin dermis

Life of this patent

Abstract

Claims5