Methods and formulations for orthopedic cell therapy
AssigneeLineage Cell Therapeutics, Inc.
InventorsMichael West, Hal Sternberg
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Abstract
Aspects of the present invention include methods and compositions related to the production and use of clonal lineages of embryonic progenitor cell lines derived from differentiating cultures of primordial stem cells, in particular, said methods and compositions relate to methods of differentiating cells in the presence of members of the BMP family of growth factors and the applications of said cell lines in the treatment of degenerative orthopedic diseases such as osteoarthritis.
Claims20
A method of increasing expression of COL2A1 and minimizing expression of COL10A1 in a clonal embryonic progenitor cell line that does not display SSEA4, TRA1-60 or TRA1-81 sero-positivity and is capable of further differentiating into more than one cell type, wherein the method comprises differentiating said clonal embryonic progenitor cell line in the presence of GDF5 or BMP6, and in the presence of TGFβ, thereby increasing the expression of COL2A1 and minimizing the expression of COL10A1 by the embryonic progenitor cell line.
A method of conditioning a clonal embryonic progenitor cell line that does not display SSEA4, TRA1-60 or TRA1-81 sero-positivity and is capable of further differentiating into more than one cell type, the method comprising culturing said clonal embryonic progenitor cell line in the presence of one or more BMP factors selected from TGF 3, GDF5, BMP2, BMP4, BMP6 and BMP7 to induce or increase expression of COL2A1, COL10A1, CRTAC1, tenomodulin, sialoprotein II, COL9A2, or CHAD by said clonal embryonic progenitor cell line.
Dependencies are inferred from the claim text.