US 10,865,381 B2 · Multi-lineage hematopoietic precursor cell production by genetic programming

Abstract

The present disclosure generally regards methods and compositions for providing multi-lineage hematopoietic precursor cells from pluripotent stem cells (PSCs). The PSCs comprise an expression construct encoding an ETS/ERG gene, GATA2 and HOXA9. Also provided are methods for providing hematopoietic stem cells capable of long-term engraftment in mammals, such as humans. Further provided are therapeutic compositions including the provided hematopoietic stem cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.

Claims35

Dependent claims

An in vitro method for producing hematopoietic precursor cells from pluripotent stem cells comprising:

(a) providing pluripotent stem cells (PSCs) comprising at least one expression construct encoding hematopoietic precursor programming genes, wherein the hematopoietic precursor programming genes comprise ETS/ERG, GATA2, and HOXA9 genes co-expressed;
(b) culturing the PSCs wherein the PSCs are induced to express the hematopoietic precursor programming genes, thereby producing hematopoietic precursor cells (HPCs);
and
(c) further culturing the HPCs, wherein expression of the hematopoietic precursor programming genes is no longer induced, to produce a population of cells comprising at least 70% multi-lineage HPCs with expression of CD34 and CD43.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/298,931Filed 20 Oct 2016 · us provisional application

Priority

20 Oct 2015

Published

US 2017/0107492 A120 Apr 2017

Granted

15 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification47 codes

C12N 5/0639
Cells from the blood or the immune system: dendritic cells, e.g. Langherhans cells in the epidermis
A61K 40/10
Cellular immunotherapy: characterised by the cell type used
A61K 40/22
Characterised by the effect or the function of the cells: immunosuppressive or immunotolerising
A61K 40/416
Vertebrate antigens: antigens related to auto-immune diseases; Preparations to induce self-tolerance

Multi-lineage hematopoietic precursor cell production by genetic programming

Life of this patent

Multi-lineage hematopoietic precursor cell production by genetic programming

Life of this patent

Abstract

Claims35