Methods and compositions for promoting survival and proliferation of endothelial cells and stimulating angiogenesis
AssigneesCornell Research Foundation, Inc., Sloan-Kettering Institute for Cancer Research
InventorsShahin Rafii, Fan Zhang, Marco Seandel
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Abstract
The present invention relates to adenovirus E4ORF1 gene and to endothelial cells engineered to express the E4ORF1 gene. The present invention also relates to uses of the E4ORF1 gene, and cells expressing the E4ORF1 gene, and to compositions comprising the E4ORF1 gene, or comprising cells expressing the E4ORF1 gene.
Claims22
A composition comprising a population endothelial cells, wherein the endothelial cells
- (a) comprise a recombinant nucleic acid sequence that comprises an E4ORF1 coding sequence under the control of a promoter, and
- (b) do not comprise nucleic acid sequences that comprise the entire adenovirus E4 region.
A method for maintaining or expanding cancer cells, stem cells or progenitor cells in vitro, the method comprising:
- a. contacting cancer cells, stem cells or progenitor cells in vitro with conditioned medium, wherein the condition medium is derived from a culture of E4ORF1-expressing endothelial cells that do not contain or express nucleic acid sequences that comprise the entire adenovirus E4 region,
- thereby maintaining or expanding the cancer cells, stem cells or progenitor cells in vitro.
A method of maintaining or expanding cancer cells, stem cells or progenitor cells in vitro, the method comprising:
- b. culturing a population of cancer cells, stem cells or progenitor cells in vitro in a culture vessel that comprises a population of E4ORF1-expressing endothelial cells, wherein the endothelial cells do not contain or express nucleic acid sequences that comprise the entire adenovirus E4 region,
- thereby maintaining or expanding the cancer cells, stem cells or progenitor cells in vitro.
Dependencies are inferred from the claim text.