Cordyceps cicadae mycelium active substances, preparation method, pharmaceutical composition and application thereof

A method of preparing a mixture of Cordyceps cicadae mycelium active substances for preventing and/or treating xerophthalmia, wherein the method comprises following steps:

• (a) culturing a Cordyceps cicadae mycelium in a plate media at 15 to 35° C. for 5 to 14 days;
• (b) inoculating the Cordyceps cicadae mycelium of step (a) to a flask containing liquid media and culturing the mycelium at 15 to 35° C. with a pH of 2 to 8 for 3 days;
• (c) inoculating the Cordyceps cicadae mycelium of step (b) to a fermenter tank and culturing the mycelium at 15 to 35° C. with a pH of 2 to 8 for 3 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid;
• (d) freeze-drying and grating the Cordyceps cicadae mycelium fermentation liquid, so as to obtain a Cordyceps cicadae mycelium powder;
• (e) dividing the Cordyceps cicadae mycelium powder into two parts, extracting one part of the Cordyceps cicadae mycelium powder with water and extracting the other part of the Cordyceps cicadae mycelium powder with alcohol separately, so as to obtain a Cordyceps cicadae mycelium water extract and a Cordyceps cicadae mycelium alcohol extract;
• and
• (f) drying the Cordyceps cicadae mycelium water extract and the Cordyceps cicadae mycelium alcohol extract, and mixing the water extracts and the alcohol extracts, so as to obtain the mixture of Cordyceps cicadae mycelium active substances.