US 10,865,373 B2 · Regenerated hair follicle primordium aggregation manufacturing method, hair follicle tissue-containing sheet, and method for manufacturing hair follicle tissue-containing sheet

Abstract

The present invention provides a method for manufacturing a regular and high-density regenerated hair follicle primordium aggregation similar to the hair follicle tissue of a mammal in a simple manner. A regenerated hair follicle primordium aggregation manufacturing method of the present invention includes a step of forming hair follicle primordia by inoculating a microwell plate, which includes regularly arranged microwell portions, with mesenchymal cells and epithelial cells and culturing a mixture of the cells while supplying oxygen thereto.

Claims8

Dependent claims

A regenerated hair follicle primordium aggregation manufacturing method, comprising:

a step of inoculating a microwell plate, which has regularly arranged microwell portions, with a mixed suspension of mesenchymal cells and epithelial cells in a medium, and culturing the mixed suspension of the mesenchymal cells and the epithelial cells suspended in the medium while supplying oxygen thereto from a surface of the microwell plate so as to form the regenerated haft follicle primordia in the microwell portions, wherein the microwell plate is formed of an oxygen permeable material.

Dependencies are inferred from the claim text.

Facts

Application

15/766,868Filed 26 Oct 2016

Priority

JP 2015-21454730 Oct 2015

Published

US 2019/0062687 A128 Feb 2019

Granted

15 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification20 codes

C12M 25/04
Membranes; Filters: in combination with well or multiwell plates, i.e. culture inserts
A61L 27/24
Polypeptides or derivatives thereof , e.g. degradation products: collagen
A61L 27/362
Characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura,…
A61L 27/3641
Containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix:…

Regenerated hair follicle primordium aggregation manufacturing method, hair follicle tissue-containing sheet, and method for manufacturing hair follicle tissue-containing sheet

Life of this patent

Regenerated hair follicle primordium aggregation manufacturing method, hair follicle tissue-containing sheet, and method for manufacturing hair follicle tissue-containing sheet

Life of this patent

Abstract

Claims8