US 10,865,372 B2 · Neuron cultivation device, neuron cultivating method, cultivated neuron, analysis and identification of protein in axon bundle, and usage of cultivated neuron

Abstract

An object is to provide a neuron cultivation device which promptly develops bundles of axons extending from neurons in vitro. A device for cultivating neuron with axon, the device comprising a cultivation plate and a plurality of modules arranged in the cultivation plate. Each of the modules includes at least one of first chambers receivable of cell bodies of neurons at least one of second chambers, and at least one of channels receivable of a bundle of axon extended from the cell bodies. The channels connect the first chambers and the second chambers. Bottom ends of the first chambers, the second chambers and the channels are closed and top ends of the first chambers and the second chambers are open.

Claims15

Dependent claims

An aggregated neuron body consisting of a plurality of neurons with cell bodies derived from human iPS cells and axons extended from the cell bodies, the aggregated neuron body comprising:

a spheroid and a bundle of axons of 1 mm or more in length extended from the spheroid in one direction,
wherein the spheroid is an aggregation of a plurality of the cell bodies, and
wherein the bundle of axons is a bundle of a plurality of the axons, glial cells being non-existent in the bundle of axons to a degree that a marker indicating existence of the glial cells is negative, wherein the marker is Glia Fibrillary Acidic Protein (GFAP).

Dependencies are inferred from the claim text.

Facts

Application

16/094,296Filed 1 Feb 2017 · us provisional application

Priority

28 Apr 2016

Published

US 2019/0127672 A12 May 2019

Granted

15 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12M 21/08
Bioreactors or fermenters specially adapted for specific uses: for producing artificial tissue or for ex-vivo cultivation of tissue
C12M 3/00
Tissue, human, animal or plant cell, or virus culture apparatus
C12M 23/12
Form or structure of the vessel: well or multiwell plates
C12M 23/16
Form or structure of the vessel: microfluidic devices; Capillary tubes

Neuron cultivation device, neuron cultivating method, cultivated neuron, analysis and identification of protein in axon bundle, and usage of cultivated neuron

Life of this patent

Neuron cultivation device, neuron cultivating method, cultivated neuron, analysis and identification of protein in axon bundle, and usage of cultivated neuron

Life of this patent

Abstract

Claims15