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US 10,865,366 B2Granted
Lipase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsCarsten Hoerslev Hansen, Jesper Vind, Luise Erlandsen, Bitten Plesner, Anna Catharina Olsen Stenholm
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Abstract
The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims13
A lipase variant, wherein the variant has lipase activity and comprises an amino acid sequence that has at least 90% but less than 100% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, and wherein the variant comprises a substitution of asparagine with glutamine at the position corresponding to position 33 of SEQ ID NO:
- 2, a substitution of glycine with glutamine at the position corresponding to position 91 of SEQ ID NO:
- 2, a substitution of glutamate with glutamine at the position corresponding to position 210 of SEQ ID NO:
- 2, a substitution of isoleucine with alanine at the position corresponding to position 255 of SEQ ID NO:
- 2, and a substitution of proline with a different amino acid at the position corresponding to position 256 of SEQ ID NO:
- 2, wherein said different amino acid is not lysine.
3 more elements
Dependencies are inferred from the claim text.