Processing cartridge and method for detecting a pathogen in a sample

A cartridge for processing a sample, the cartridge comprising:

• a first substrate comprising:
• a sample well,
• a pump port, and
• a deformable fluid chamber in fluid communication with the sample well;
• a second substrate comprising:
• a microfluidic network for receiving a processed sample from the first substrate, and
• electrowetting pads;
• and
• an interstitial space between the first substrate and second substrate, wherein the interstitial space forms a void.

A cartridge for processing a sample, the cartridge comprising:

• a first substrate comprising:
• a sample well, and
• a pump port, wherein fluid moves out of the sample well only via pressure applied through the pump port;
• and
• a second substrate to receive the fluid from the first substrate, the second substrate comprising:
• electrowetting pads configured to move the fluid in the second substrate, wherein the fluid is moved in the second substrate only via digital microfluidics on the electrowetting pads.