Chromatography matrix
AssigneeCytiva BioProcess R&D AB
InventorsJean-Luc Maloisel, Tobias Söderman, Ola Lind, Ida Eklind
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Abstract
The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.
Claims33
A separation matrix for purification of biomacromolecules, comprising a plurality of particles having a core region and a shell region, wherein:
- a) said shell region is accessible to a target biomacromolecule, wherein said shell region does not comprise polymers grafted to a porous support material and does not comprise polymers grafted to said shell;
- b) said core region is less accessible to said target biomacromolecule than said shell region;
- and
- c) said core region comprises at least 50 mg/ml of a vinyl polymer grafted to the porous support material and comprising residues of at least one polymerizable monomer, wherein said vinyl polymer comprises vinyl amide monomer residues selected from the group consisting of N-vinyl pyrrolidone, N-vinyl caprolactam, N-vinyl formamide and N-vinyl acetamide.
Dependencies are inferred from the claim text.