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US 10,864,483 B2Granted
Molecular weight filtration system and apparatus
AssigneeIntegrated Protein Technologies, Snc.
InventorsPhilip D. Compton, Jared Drader
Life of this patent
Estimating the term…
Abstract
A molecular filtration device and method of use capable of filtering and purifying molecules of a particular characteristic, wherein the amount of molecule to be filtered may be in the nanogram range and may be dispersed in a relatively large volume of solution. The resultant elution may include a substantially pure solution comprising the desired molecule.
Claims20
A molecular filtration device comprising:
- an upper portion;
- and
- a lower portion;
- wherein said upper portion comprises two upper ports;
- wherein said two upper ports comprise a first upper port and a second upper port;
- wherein said first upper port is configured to receive a first upper flow device;
- wherein said second upper port is configured to receive a second upper flow device;
- wherein said first upper flow device is configured to alternate between injecting and not injecting a solution;
- wherein said second upper flow device is configured to alternate between injecting and withdrawing the solution;
- wherein said lower portion comprises a lower port and a reservoir;
- wherein said lower port is configured to receive a lower flow device;
- wherein said lower flow device is configured to alternate between injecting and withdrawing the solution from said reservoir;
- wherein said upper portion comprises a channel forming lip;
- wherein a channel forming cavity is formed by said channel forming lip when said upper portion and said lower portion engage one another;
- wherein a lower sealing surface of said upper portion and an upper sealing surface of said lower portion are configured to receive and compress a membrane;
- wherein said membrane is configured to extend beyond an outer edge of said channel forming lip, wherein a portion of said membrane that is located within an inner edge of said channel forming lip is not compressed;
- wherein a channel is defined by said channel forming cavity and said membrane.
14 more elements
A method for automated molecular sample analysis comprising the steps;
- introducing a sample into a molecular purification system;
- wherein said sample comprises at least one type of molecule to be isolated;
- wherein said molecular purification system comprises one or more molecular filtration devices;
- wherein said one or more molecular filtration devices comprise an upper portion and
- a lower portion;
- wherein said upper portion comprises two upper ports;
- wherein said two upper ports comprise a first upper port and a second upper port;
- wherein said first upper port is configured to receive a first upper flow device;
- wherein said second upper port is configured to receive a second upper flow device;
- wherein said first upper flow device is configured to alternate between injecting and not injecting a solution;
- wherein said second upper flow device is configured to alternate between injecting and withdrawing the solution;
- wherein said lower portion comprises a lower port and a reservoir;
- wherein said lower port is configured to receive a lower flow device;
- wherein said lower flow device is configured to alternate between injecting and withdrawing the solution from said reservoir;
- wherein said upper portion comprises a channel forming lip;
- wherein a channel forming cavity is formed by said channel forming lip when said upper portion and said lower portion engage one another;
- wherein a lower sealing surface of said upper portion and an upper sealing surface of said lower portion are configured to receive and compress a membrane;
- wherein said membrane is configured to extend beyond an outer edge of said channel forming lip, wherein a portion of said membrane that is located within an inner edge of said channel forming lip is not compressed;
- wherein a channel is defined by said channel forming cavity and said membrane;
- wherein said sample is loaded into said one or more molecular filtration devices.
17 more elements
A molecular filtration device comprising:
- an upper portion;
- and
- a lower portion;
- wherein said upper portion comprises two upper ports;
- wherein said two upper ports comprise a first upper port and a second upper port;
- wherein said first upper port is configured to receive a first upper flow device;
- wherein said second upper port is configured to receive a second upper flow device;
- wherein said first upper flow device is configured to alternate between injecting and not injecting a solution;
- wherein said second upper flow device is configured to alternate between injecting and withdrawing the solution;
- wherein said lower portion comprises a lower port and a reservoir;
- wherein said lower port is configured to receive a lower flow device;
- wherein said lower flow device is configured to alternate between injecting and withdrawing the solution from said reservoir;
- wherein said upper portion comprises a channel forming lip;
- wherein a channel forming cavity is formed by said channel forming lip when said upper portion and said lower portion matingly engage one another;
- wherein a lower sealing surface of said upper portion and an upper sealing surface of said lower portion are configured to receive and compress a membrane;
- wherein said membrane is configured to extend beyond an outer edge of said channel forming lip, wherein a portion of said membrane that is located within an inner edge of said channel forming lip is not compressed;
- wherein a channel is defined by said channel forming cavity and said membrane;
- wherein said filtration membrane is a molecular weight cut off filtration membrane;
- wherein said upper sealing surface of said lower portion and said lower sealing surface of said upper portion are configured to apply a pressure to one another through said membrane;
- wherein said channel has a volume of between 5 uL and 50 uL;
- wherein said reservoir comprises a frit supporting lip;
- wherein said device further comprises a frit;
- wherein said frit supporting lip is configured to receive said frit;
- wherein said frit comprises a porous structure;
- and
- wherein said frit is rigid.
23 more elements
Dependencies are inferred from the claim text.