Methods of purifying cannabinoids, compositions and kits thereof

A method of purifying a cannabinoid from a plant material, the method consisting essentially of the following steps:

• a) incubating the plant material with a solvent selected from the group consisting of pentane, hexane, heptane, cyclohexane, petroleum ethers, dichloromethane, trichloromethane, tethrahydrofuran, diethyl ether, ethanol, methanol, isopropanol, acetone, acetonitrile, ethyl acetate, butane, propane, refrigerant gas 1,1,1,2-Tetrafluoroethane (R134a), liquid CO2, subcritical CO2 or supercritical CO2 or mixes of these solvents to form a first solvent mixture which extracts the one or more cannabinoids from a plant material;
• b) filtering, decanting or centrifuging the first solvent mixture;
• c) reducing the volume of the first solvent mixture to dryness thereby concentrating the one or more cannabinoids in a first extract;
• d) incubating the first extract with a non-polar solvent selected from the group consisting of petroleum ether, pentane, hexane and heptane to form a second solvent mixture which extracts the one or more cannabinoids from the first extract;
• e) filtering, decanting or centrifuging the second solvent mixture;
• f) incubating the second solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystalize the one or more cannabinoids;
• g) collecting the one or more crystalized cannabinoids in step (f) using filtration that results in a collection of a crystalline product and a mother liquor;
• h) incubating the one or more crystalized cannabinoids of step (g) with the non-polar solvent wherein the non-polar solvent is the same non-polar solvent as in step (d) to form a third solvent mixture, wherein the third solvent mixture dissolves at least 50% of the one or more crystalized cannabinoids;
• i) incubating the third solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystalize the one or more cannabinoids, and then washing the crystallized one or more cannabinoids by dissolving in ethanol, methanol, isopropyl alcohol or acetone, filtering, evaporating to dryness and recrystallizing using the same non-polar solvent as in step d);
• and
• j) collecting the one or more crystalized cannabinoids of step (i) using filtration that results in a collection of a crystalline product and a mother liquor, thereby resulting in the purification of one or more cannabinoids.