Method for preparing bilayer scaffold through single process and method for regenerating tissue using bilayer scaffold obtained by preparing method

A method for preparing a bilayer scaffold through single process, comprising:

• preparing a gelatin aqueous solution by stirring gelatin in water at a temperature of 60 to 70° C. so as to dissolve completely;
• preparing a polymer blend solution by adding polyvinyl alcohol into the gelatin aqueous solution and stirring to create a polymer blend solution;
• adding sodium dodecyl sulfate into the polymer blend solution and stirring at a temperature of 80 to 120° C.;
• freeze-drying the stirred solution thereby obtaining a sponge;
• dipping the sponge in a cross-linking agent selected from the group consisting of glutaraldehyde or ethyldiethylaminopropylcarbodimide thereby rendering the sponge cross-linked;
• and
• freeze-drying the cross-linked sponge,
• wherein the gelatin aqueous solution includes 2.5% by weight of gelatin,
• wherein the polymer blend solution includes 10% by weight of polyvinylalcohol.