Back to results
US 10,864,264 B2Granted
Influenza virus reassortment
AssigneesSeqirus UK Limited, Synthetic Genomics, Inc.
InventorsPhilip Dormitzer, Peter Mason, Pirada Suphaphiphat, Raul Gomila
Life of this patent
Estimating the term…
Abstract
New influenza donor strains for the production of reassortant influenza B viruses are provided.
Claims42
A method of preparing a reassortant influenza B virus comprising steps of:
- (i) introducing into a culture host one or more expression construct(s) which encode(s) the viral segments required to produce an influenza B virus wherein the expression construct(s) encode the HA segment from a first influenza B virus and the NP and/or PB2 segment from a second influenza B virus which is a B/Victoria/2/87-like strain;
- and
- (ii) culturing the culture host in order to produce a reassortant influenza B virus.
A method of preparing a reassortant influenza B virus comprising steps of:
- (i) introducing into a culture host one or more expression construct(s) which encode(s) the viral segments required to produce an influenza B virus wherein the expression construct(s) encode the HA segment from a first influenza B virus and the NP segment from a second influenza B virus which is not B/Lee/40 or B/Ann Arbor/1/66 or B/Panama/45/90;
- and
- (ii) culturing the culture host in order to produce a reassortant influenza B virus.
A method of preparing a reassortant influenza B virus comprising steps of
- (i) introducing into a culture host one or more expression construct(s) which encode(s) the viral segments required to produce an influenza B virus comprising the HA segment from a B/Yamagata/16/88-like strain and at least one backbone segment from a B/Victoria/2/87-like strain;
- and
- (ii) culturing the culture host in order to produce a reassortant influenza B virus.
A method of preparing a reassortant influenza B virus comprising steps of
- (i) introducing into a culture host one or more expression construct(s) which encode(s) the viral segments required to produce an influenza B virus comprising viral segments from a B/Victoria/2/87-like strain and a B/Yamagata/16/88-like strain, wherein the ratio of segments from the B/Victoria/2/87-like strain and the B/Yamagata/16/88-like strain is 1:7, 2:6, 3:5, 4:4, 5:3, 6:2 or 7:1;
- and
- (ii) culturing the culture host in order to produce a reassortant influenza B virus.
A method of preparing a reassortant influenza B virus comprising steps of
- (i) introducing into a culture host one or more expression construct(s) which encode(s) the viral segments required to produce an influenza B virus comprising:
- a) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 16 and the M segment of SEQ ID NO:
- 15;
- or
- b) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 31, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34;
- or
- c) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 31, the PB2 segment of SEQ ID NO:
- 32, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 16 and the M segment of SEQ ID NO:
- 15;
- or
- d) the PA segment of SEQ ID NO:
- 30, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 16 and the M segment of SEQ ID NO:
- 15, or
- e) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34;
- f) the PA segment of SEQ ID NO:
- 30, the PB1 segment of SEQ ID NO:
- 31, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 33, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34, or
- g) the PA segment of SEQ ID NO:
- 30, the PB1 segment of SEQ ID NO:
- 31, the PB2 segment of SEQ ID NO:
- 32, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34, or
- h) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 33, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34, or
- i) the PA segment of SEQ ID NO:
- 11, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 32, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34, or
- j) the PA segment of SEQ ID NO:
- 30, the PB1 segment of SEQ ID NO:
- 12, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34, or
- k) the PA segment of SEQ ID NO:
- 30, the PB1 segment of SEQ ID NO:
- 31, the PB2 segment of SEQ ID NO:
- 13, the NP segment of SEQ ID NO:
- 14, the NS segment of SEQ ID NO:
- 35 and the M segment of SEQ ID NO:
- 34;
- and
- (ii) culturing the culture host in order to produce a reassortant influenza B virus.
80 more elements
Dependencies are inferred from the claim text.