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US 10,863,729 B2Granted
Rodents having a humanized TMPRSS gene
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLisa Purcell, Alexander O. Mujica, Yajun Tang
Life of this patent
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Abstract
Genetically modified rodents such as mice and rats, and methods and compositions for making and using the same, are provided. The rodents comprise a humanization of at least one endogenous rodent Tmprss gene, such as an endogenous rodent Tmprss2, Tmprss4, or Tmprss11d gene.
Claims16
A rodent embryonic stem (ES) cell, comprising a replacement of a nucleotide sequence of an endogenous rodent Tmprss2 gene at an endogenous Tmprss2 locus with a nucleotide sequence of a human TMPRSS2 gene to form a humanized Tmprss2 gene,
- wherein the humanized Tmprss2 gene is under control of the promoter of the endogenous rodent Tmprss2 gene at the endogenous Tmprss2 locus, and
- wherein the humanized Tmprss2 gene encodes a humanized Tmprss2 protein that comprises (i) an ectodomain of the human TMPRSS2 protein encoded by the human TMPRSS2 gene, and (ii) a cytoplasmic and transmembrane portion encoded by the endogenous rodent Tmprss2 gene.
Dependencies are inferred from the claim text.