Biochemical-immunological hybrid biosensor and sensor system including the same

A biochemical-immunological hybrid biosensor comprising a reaction strip in the form of a porous membrane through which a sample moves by capillary action wherein the reaction strip comprises:

• a sample transfer pad having a predetermined length and along which the sample is transferred from one end to the other in the lengthwise direction;
• a sample addition pad absorbing the sample loaded from the outside;
• a conjugate pad comprising detection antibodies specifically binding to protein markers in the sample and a tracer generating color signals for immunoreactions with the protein markers, and connecting one end of the sample transfer pad to the sample addition pad;
• one or more immunoreaction zones, formed on the sample transfer pad, comprising capture antibodies specifically binding to the protein markers;
• at least one biochemical reaction zone comprising a chromogenic substrate generating a color signal for a biochemical reaction and at least one enzyme producing a product reacting with the chromogenic substrate from a biochemical marker in the sample which is received from at least one of the sample transfer pad, the sample addition pad, and the conjugate pad, and where a biochemical reaction occurs independently from the reactions in the immunoreaction zones;
• a sample absorption pad arranged at the other end of the sample transfer pad to absorb the sample transferred along the sample transfer pad;
• and at least one biochemical reaction pad extending outward from at least one of the sample transfer pad, the sample addition pad, and the conjugate pad, and the biochemical reaction zone is arranged on one side of the biochemical reaction pad,
• wherein the detection antibodies are conjugated with the tracer,
• wherein some of the sample are transferred to the sample transfer pad through the conjugate pad, and some of the sample are transferred to the biochemical reaction pad,
• wherein the protein markers bound the detection antibodies conjugated with the tracer generating color signals are bound to the capture antibodies, so that the color signals for immunoreactions are generated in the immunoreaction zones,
• wherein the biochemical marker in the sample reacts with the enzyme and the chromogenic substrate, so that the color signal for the biochemical reaction is generated in the biochemical reaction zone.