US 10,788,484 B2 · Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis

Abstract

Provided is a method for quantitative analysis of a sample. The method includes steps of (a) providing a singular marker representative of one or more features of the sample, the sample comprising a population of individual units of the marker; (b) measuring the marker with dot blot analysis, wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the sample volume or by the sample weight; and (c) obtaining an objective determination of the one or more features of the sample based on the quantitation result of the marker. Also disclosed is a reference database and a method to use the reference database for diagnosing cancer in a patient.

Claims13

Dependent claims

A method for quantitative analysis of a sample, comprising:

(a) providing a singular marker representative of one or more features of the sample, the sample being a formalin-fixed paraffin-embedded (FFPE) tissue sample having a population of individual units of the marker;
(b) extracting markers from the tissue sample and measuring the marker with quantitative dot blot analysis (QDB), wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the protein weight, and the marker standard curve is established to convert signals captured into absolute marker level;
and (c) obtaining a determination of the one or more features of the sample based on the absolute quantitation result of the marker.

Dependencies are inferred from the claim text.

Facts

Application

16/022,259Filed 28 Jun 2018 · us provisional application

Priority

29 Jun 2017

Published

US 2019/0004037 A13 Jan 2019

Granted

29 Sep 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

G01N 33/54306
With an insoluble carrier for immobilising immunochemicals: solid-phase reaction mechanisms
G01N 33/575
Immunoassay; Biospecific binding assay; Materials therefor: for cancer
G01N 33/5758
For cancer: involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress…
G01N 33/6893
Involving proteins, peptides or amino acids: related to diseases not provided for elsewhere

Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis

Life of this patent

Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis

Life of this patent

Abstract

Claims13