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US 10,768,402 B2Granted
Microscopy of a tissue sample using structured illumination
AssigneeThe Administrators of the Tulane Educational Fund
InventorJonathon Quincy Brown
Life of this patent
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Abstract
Systems and methods are provided for evaluating a fresh tissue sample, prepared as to fluoresce under illumination, during a medical procedure. A structured light source is configured to project a spatially patterned light beam onto the fresh tissue sample. An imaging system is configured to produce an image from fluorescence emitted from the illuminated fresh tissue sample. A system control is configured to provide a human-comprehensible clinically useful output associated with the medical procedure.
Claims15
A method comprising:
- projecting light through a spatial light modulator in a plurality of orientations to provide a plurality of spatial light patterns sequentially illuminating a focal plane within an excised tissue sample, wherein the tissue sample is pre-stained with a fluorescent contrast agent;
- imaging the fluorescence emitted from the sample as each of the plurality of spatial light patterns are projected on the tissue sample to form a plurality of microscopy images;
- and
- combining the plurality of microscopy images to form an image of an optical section of the excised tissue sample, wherein the image exhibits an improved contrast due to rejecting out of focus light in the plurality of microscopy images during the combining.
1 more element
A system comprising:
- a light source to project a light beam onto an excised tissue sample;
- a spatial light modulator to modulate the light beam, wherein the spatial light modulator is arranged in a plurality of orientations to form a plurality of illumination patterns onto a focal plane of the excised tissue sample;
- and
- an imaging system to produce a plurality of microscopy images from fluorescence emitted from the tissue sample due to a plurality of illumination patterns, wherein the plurality of microscopy images are combined to provide an image of an optical section of the excised tissue sample with an improved contrast, wherein the improved contrast is provided by rejecting out of focus light in the plurality of microscopy images.
1 more element
Dependencies are inferred from the claim text.