Method for analyzing an NMR spectrum of a lipoprotein-containing sample

A method for analyzing an NMR spectrum of a lipoprotein-containing sample, comprising the following steps:

• a) defining a spectral range to be analyzed of at least 0.5 ppm of an NMR spectrum of a lipoprotein-containing sample,
• b) fitting a first spectral function of a first individual lipid component and at least one further spectral function of a further individual lipid component into the whole spectral range to be analyzed,
• wherein the first spectral function represents at least a part of an NMR spectrum of the first individual lipid component taking into account the behavior of the first lipid component in the surroundings of a lipoprotein particle of a first defined lipoprotein class or lipoprotein subclass, and
• wherein the further spectral function represents at least a part of an NMR spectrum of the further individual lipid component taking into account the behavior of the further lipid component in the surroundings of a lipoprotein particle of the same defined lipoprotein class or lipoprotein subclass, and
• c) determining a concentration or an amount of the first individual lipid component and the further individual lipid component and therefrom a concentration or an amount of the first defined lipoprotein class or lipoprotein subclass in the lipoprotein-containing sample and optionally of each other defined lipoprotein class or lipoprotein subclass based on the fitting operations of step b).