Method for detecting nucleosomes containing histone modifications and variants
AssigneeBelgian Volition SPRL
InventorsMark Edward Eccleston, Jacob Vincent Micallef
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Abstract
The present invention relates to a method for detecting and measuring the presence of cell free mono-nucleosomes and oligo-nucleosomes that contain histone H1 or a histone H1 modification, variant or isoform, and the use of such measurements for the detection and diagnosis of disease.
Claims8
A method for detecting the presence of a cell free nucleosome containing histone H1 or a histone H1 modification, variant or isoform in a sample which comprises the steps of:
- (i) contacting the sample with a first binding agent which binds to a non-histone H1 nucleosome epitope;
- (ii) contacting the nucleosomes or sample with a second binding agent which binds to histone H1 or the histone H1 modification, variant or isoform;
- (iii) detecting or quantifying the binding of said second binding agent to histone H1 or the histone H1 modification, variant or isoform in the sample;
- and
- (iv) using the presence or degree of binding of said second binding agent as a measure of the presence of nucleosomes containing histone H1 or the histone H1 modification, variant or isoform in the sample.
A method for detecting the presence of a cell free nucleosome containing histone H1 or a histone H1 modification, variant or isoform in a sample which comprises the steps of:
- (i) contacting the sample with a first binding agent which binds to histone H1 or the histone H1 modification, variant or isoform;
- (ii) contacting the nucleosomes or sample with a second binding agent which binds to a non-histone H1 nucleosome epitope;
- (iii) detecting or quantifying the binding of said second binding agent to the non-histone H1 nucleosome epitope in the sample;
- and
- (iv) using the presence or degree of binding of said second binding agent as a measure of the presence of nucleosomes containing histone H1 or the histone H1 modification, variant or isoform in the sample.
Dependencies are inferred from the claim text.