Detection of an antibody against a pathogen
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsStephen J. Elledge, Harry B. Larman, Tomasz Kula, George Xu
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Abstract
Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof. One aspect provided herein relates to a method for detecting an antibody against a pathogen in a subject, the method comprising: (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library comprises a plurality of peptides derived from a plurality of pathogens, and (b) detecting a peptide bound to at least one antibody, thereby detecting an antibody capable of binding the peptide.
Claims5
A method for detecting the presence of an antibody against a pathogen in a subject, the method comprising:
- (a) contacting a reaction sample comprising a recombinant phage display library with a biological sample comprising antibodies, wherein the phage display library comprises a plurality of peptides derived from at least three unique pathogens
- wherein the phage display library is constructed using a synthetic, programmable microarray from a starting library that is uniformly distributed and
- (b) lysing the phage display library and amplifying DNA therefrom,
- (c) detecting at least one antibody bound to at least one peptide expressed by the phage display library, thereby detecting the presence of an antibody against the at least one pathogen peptide in the biological sample.
Dependencies are inferred from the claim text.