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US 10,768,178 B2Granted
Compositions and methods for improved cell-based botulinum neurotoxin assays
AssigneeBioMadison, Inc.
InventorsWard C. Tucker, Timothy M. Piazza
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Abstract
Methods for a cell-based assay for Botulinum neurotoxin are provided in which a transfected cell that produces a reporting peptide is contacted with a Botulinum neurotoxin in media having a sub-physiological osmolarity and a temperature that is above physiological temperature. This combination provides an unexpected synergistic effect in reducing the EC50 of the cell-based assay relative to an analogous cell-based assay performed at physiological osmolarity and temperature.
Claims7
A method of increasing sensitivity of a cell based assay for Botulinum neurotoxin, comprising:
- (i) providing, in a media having a sub-physiological osmolarity and a temperature of at least 39° C., a transfected cell comprising a reporting peptide cleavable by Botulinum neurotoxin;
- (ii) contacting the cell with Botulinum neurotoxin;
- and
- (iii) measuring a cleavage product derived from the reporting peptide,
- thereby providing a synergistic decrease in EC50 for Botulinum neurotoxin relative to the cell-based assay performed at physiological temperature and physiological osmolarity.
2 more elements
Dependencies are inferred from the claim text.