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US 10,768,176 B2Granted
Hetero functional binding systems
AssigneeAnteo Technologies Pty Ltd
InventorsChang-yi Huang, Nobuyoshi Joe Maeji
Life of this patent
Estimating the term…
Abstract
The present invention relates to reagents and methods for binding compounds to surfaces that are hydrophobic. More specifically, the invention relates to simple methods for coating of hydrophobic planar, membrane or particle surfaces to facilitate binding of molecules such as labels, dyes, synthetic and biological polymers and/or nanoparticles thereto.
Claims12
A modified substrate for binding of a target molecule thereon, the substrate including:
- a surface that is hydrophobic wherein the substrate is composed of a metal, a metal or metalloid composite, a synthetic polymer, a plastic, or carbon, and
- an oligomeric chromium complex layer having:
- (i) one or more chromium co-ordination sites occupied by a hydrophobic ligand for binding the oligomeric chromium complex layer to the hydrophobic surface wherein the hydrophobic ligand is of the form R—X, where X is independently selected from a carboxylic acid, aldehyde, polyalcohol, sulfonic acid, phosphonic acid, phosphate and bisulfate group, and R is independently selected from alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, alkylcycloalkyl, heteroalkylcycloalkyl, aryl, heteroaryl, aralkyl and heteroaralkyl groups which groups are optionally substituted and wherein the degree to which R—X coordinates with the oligomeric chromium complex layer is in the range of about 25% to about 75%, and
- (ii) one or more chromium co-ordination sites available for binding to a target molecule,
- wherein the hydrophobic ligand binds to the hydrophobic surface by non-covalent and non-coordinative interactions such that the co-ordination sites available for binding to a target molecule are directed away from the hydrophobic surface.
2 more elements
A method of modifying a hydrophobic surface, the method including:
- providing a surface that is hydrophobic, wherein the surface is a surface of a substrate which is composed of a metal, a metal or metalloid composite, a synthetic polymer, a plastic, or carbon, and
- contacting the surface with an oligomeric chromium complex layer having:
- (i) one or more chromium co-ordination sites occupied by a hydrophobic ligand for binding the oligomeric chromium complex layer to the hydrophobic surface wherein the hydrophobic ligand is of the form R—X, where X is independently selected from a carboxylic acid, aldehyde, polyalcohol, sulfonic acid, phosphonic acid, phosphate and bisulfite group, and R is independently selected from alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, alkylcycloalkyl, heteroalkylcycloalkyl, aryl, heteroaryl, aralkyl and heteroaralkyl groups which groups are optionally substituted and wherein the degree to which R—X coordinates with the oligomeric chromium complex layer is in the range of about 25% to about 75%, and
- (ii) one or more chromium co-ordination sites available for binding to a target molecule,
- wherein the hydrophobic ligand binds to the hydrophobic surface by non-covalent and non-coordinative interactions such that the co-ordination sites available for binding to a target molecule are directed away from the hydrophobic surface, thereby forming a modified surface having increased hydrophilicity.
2 more elements
A particle, the particle including:
- a surface that is hydrophobic, wherein the surface is a surface of a substrate which is composed of a metal, a metal or metalloid composite, a synthetic polymer, a plastic, or carbon, and
- an oligomeric chromium complex layer having:
- (i) one or more chromium co-ordination sites occupied by a hydrophobic ligand for binding the oligomeric chromium complex layer to the hydrophobic surface wherein the hydrophobic ligand is of the form R—X, where X is independently selected from a carboxylic acid, aldehyde, polyalcohol, sulfonic acid, phosphonic acid, phosphate and bisulfate group, and R is independently selected from alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, alkylcycloalkyl, heteroalkylcycloalkyl, aryl, heteroaryl, aralkyl and heteroaralkyl groups which groups are optionally substituted and wherein the degree to which R—X coordinates with the oligomeric chromium complex layer is in the range of about 25% to about 75%, and
- (ii) one or more chromium co-ordination sites available for binding to a target molecule,
- wherein the hydrophobic ligand binds to the hydrophobic surface by non-covalent and non-coordinative interactions such that the co-ordination sites available for binding to a target molecule are directed away from the hydrophobic surface.
2 more elements
Dependencies are inferred from the claim text.