Method for tracing wine origin based on multielements and stable isotopes

A method for tracing wine origin based on multielements and stable isotopes, characterized by comprising steps of:

• 1) sample collection:
• collecting wine samples from a plurality of origins;
• 2) sample pre-processing:
• filtering each of the wine samples collected in Step 1) using a pore size of 0.22 μm aqueous filter membrane, and pouring 1.5 ml of a filtrate into an autosampler vial for carbon stable isotope ratio analysis of ethanol and glycerol;
• pouring 0.3 ml of the wine sample into a 12 ml glass tube with a stopper for analyzing oxygen stable isotopes in water;
• and pouring 0.5 ml of the wine sample into a 15 ml centrifuge tube, and adding 0.5 ml of concentrated HNO3, performing digestion overnight, and diluting to a constant volume of 10 ml to be tested;
• 3) sample analysis:
• performing carbon stable isotope analysis, oxygen stable isotope ratio analysis and elemental analysis, respectively;
• 4) statistical modeling:
• utilizing information of the origins of the wine samples and analytical data of Step 3) to perform statistical modeling to obtain a origin discrimination model coefficient matrix and a corresponding predicted accuracy rate of the origin discrimination;
• and
• 5) sample testing:
• performing origin discrimination by using an unknown sample in the model, and ultimately determining attributes of the origin.