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US 10,768,141 B2Granted
Isoelectric focusing arrays and methods of use thereof
AssigneeThe Regents of the University of California
InventorsAmy E. Herr, Augusto M. Tentori, Kevin A. Yamauchi
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Abstract
Isoelectric focusing devices configured for multiplex separation of sample components of interest in a polymeric separation medium are provided. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.
Claims23
A device comprising:
- a polymeric separation medium comprising two or more isoelectric focusing regions each comprising an acidic boundary sub-region, a basic boundary sub-region, and a focusing sub-region positioned between the acidic boundary sub-region and the basic boundary sub-region,
- wherein each isoelectric focusing region is configured for multiplex separation of sample components of interest from two or more samples in the polymeric separation medium,
- wherein the two or more isoelectric focusing regions are arranged in series along an axis of the polymeric separation medium, and
- wherein each isoelectric focusing region comprises a pH gradient along the axis of the polymeric separation medium.
1 more element
Dependencies are inferred from the claim text.