In-vitro biological valve calcification evaluation method and reducing calcium ingredient solution

An in-vitro biological valve calcification evaluation method, comprising the steps of:

• (1) preparing calcification evaluation solutions, the calcification evaluation solutions comprising:
• glutaraldehyde solution, saline solution, borate buffer solution, reducing calcium ingredient solution and calcium-containing plasma solution, wherein the reducing calcium ingredient solution comprises an alcohol, an alkaline solution, an oil and/or its derivatives, and a buffer solution;
• (2) preheating the reducing calcium ingredient solution by placing the reducing calcium ingredient solution in a 30 to 40° C. thermostat water bath;
• (3) conducting treatment prior to reducing calcium ingredient solution by taking a biological valve sample and soaking the biological valve sample in the glutaraldehyde solution completely, and maintaining the biological valve sample at room temperature;
• (4) conducting treatment with the reducing calcium ingredient solution by removing the glutaraldehyde solution, and adding the borate buffer solution, immersing the sample therein, then removing the borate buffer solution, and soaking the sample in the reducing calcium ingredient solution completely within a 30 to 40° C. thermostat water bath;
• (5) preserving the biological valve samples by removing the reducing calcium ingredient solution from a container and adding the saline solution until the biological valve sample is completely soaked, and storing the sample in the container;
• (6) conducting an in-vitro calcification reaction by putting the biological valve sample into the calcium-containing plasma solution in a 30 to 40° C. thermostat water bath for a period of any days within 60 days to let the biological valve sample fully react with the given calcium ions of the solution, such that calcium ions are bonded to the biological valve sample by chemical bonding ;
• (7) detecting calcium content measuring the calcium content of the biological reacted valve sample by atomic absorption spectrometry to evaluate the calcium content of the biological valve sample, and comparing it with a standard sample.