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US 10,767,278 B2Granted
Chemically encoded spatially addressed library screening platforms
AssigneeCity of Hope
InventorsJacob Berlin, Gregory Copeland, Kathleen Elison, Hurik Muradyan
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Abstract
Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.
Claims28
A method comprising:
- (i) attaching a microparticle to a solid support, thereby forming an immobilized microparticle, wherein said immobilized microparticle is further covalently attached to:
- (a) a ligand domain through a first linker;
- and
- (b) a nucleic acid domain through a second linker;
- wherein said second linker comprises an acyl group, a sulfonamide group, or a photocleavable group, and
- wherein said second linker is cleavable and said first linker is not cleavable under a condition that said second linker is cleavable;
- and
- (ii) performing a decoding procedure on the nucleic acid domain, thereby identifying the composition of the ligand domain and its location on said solid support.
5 more elements
A method comprising:
- (i) providing a solid support comprising a plurality of wells;
- (ii) attaching a plurality of microparticles to said plurality of wells to form an ordered array of a plurality of immobilized microparticles, wherein each of said plurality of immobilized microparticles is further covalently attached to:
- (a) a ligand domain through a first linker;
- and
- (b) a nucleic acid domain through a second linker;
- wherein said second linker comprises an acyl group, a sulfonamide group, or a photocleavable group, and
- wherein said second linker is cleavable and said first linker is not cleavable under a condition that said second linker is cleavable;
- and
- (iii) performing a decoding procedure on the nucleic acid domain of each of said plurality of microparticles, thereby identifying the composition of the corresponding ligand domain and its location on said solid support.
6 more elements
Dependencies are inferred from the claim text.