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US 10,767,221 B2Granted
Enzyme-linked nucleotides
AssigneeIllumina, Inc.
InventorsChristian Gloeckner, Matthew William Kellinger, Lea Pickering
Life of this patent
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Abstract
Presented herein are polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules. Also presented are methods and systems using the polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules.
Claims8
A modified DNA polymerase comprising:
- a) a point mutation wherein a surface-exposed amino acid residue is replaced with a cysteine residue;
- b) a flexible linker having a first end and second end, the first end covalently attached to the cysteine residue;
- c) a nucleotide attached to the second end of the flexible linker, wherein the flexible linker attachment to the nucleotide allows, the nucleotide to interact at a catalytically active region of said modified DNA polymerase and incorporate the nucleotide to the 3′ end of a polynucleotide in the presence of a template nucleic acid sequence thereby producing a detectable increase in dwell time at the site of incorporation;
- and
- c) an optically detectable label comprising a fluorophore attached to the modified DNA polymerase.
2 more elements
Dependencies are inferred from the claim text.