Method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction
AssigneeRoche Molecular Systems, Inc.
InventorAlan Furlan
Life of this patent
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Abstract
The present disclosure relates to a method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction (dPCR) and to a method for determining the amount or concentration of a nucleic acid of interest in a sample with dPCR.
Claims20
A method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction (dPCR), wherein the amount or concentration of a nucleic acid of interest is quantified in an array of reaction areas, the method comprising
- a) adding a fill control marker to each reaction area of the array of reaction areas used in dPCR;
- b) quantifying the reaction volume in each reaction area;
- and
- c) calculating the amount or concentration of the nucleic acid of interest as a number of nucleic acid(s) as determined by dPCR in a volume or per volume, wherein the volume is the sum of the reaction volumes as determined in step b).
A method for determining the amount or concentration of a nucleic acid of interest in a sample, the method comprising the steps of:
- a) providing a sample suspected of containing the nucleic acid of interest;
- b) performing a dPCR with the sample in each reaction area of an array of reaction areas;
- c) quantifying the reaction volume in each reaction area;
- and
- d) calculating the amount or concentration of the nucleic acid of interest as a number of nucleic acid(s) as determined in step b) in a volume or per volume, wherein the volume is the sum of the reaction volumes as determined in step c).
Dependencies are inferred from the claim text.