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US 10,767,217 B2Granted
Compositions, methods and kits for real time polymerase chain reaction (PCR)
AssigneeLife Technologies Corporation
InventorsCarole Bornarth, Michael Lau, Junko Stevens
Life of this patent
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Abstract
The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.
Claims14
A method for amplifying a target nucleic acid comprising:
- a) contacting the target nucleic acid with a first nucleic acid polymerase, a dual hot start reaction mixture, at least one primer and at least one dNTP at a first temperature, thereby forming a reaction composition, wherein the dual hot start reaction mixture comprises at least two hot start mechanisms that, together, both inhibit activity of the first nucleic acid polymerase;
- b) heating the reaction composition to a second temperature;
- and
- c) amplifying the target nucleic acid in the reaction composition.
1 more element
Dependencies are inferred from the claim text.