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US 10,767,215 B2Granted
Stabilizing compositions and methods for extraction of ribonucleic acid
AssigneeDna Genotek Inc.
InventorsHyman Chaim Birnboim, Adele Jackson
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Abstract
The present invention provides a composition and method for stabilizing ribonucleic acid (RNA) from biological samples such that the ribonucleic acid within the sample remains stable at room temperature. The composition comprises an anionic detergent and a buffering agent at a pH of about 5 to about 8.2 and is used in methods for extracting and storing ribonucleic acid from the biological sample.
Claims19
A phenol-free method for preserving ribonucleic acid from a biological sample comprising the steps of:
- a. obtaining the sample from a subject;
- b. contacting the sample with a composition comprising an anionic detergent, wherein the anionic detergent is at a concentration of 1% to 8%, and a buffering agent at a pH of about 5 to about 8.2 to form a mixture;
- and
- c. storing the mixture at room temperature;
- wherein the composition stabilizes the ribonucleic acid at room temperature.
2 more elements
Dependencies are inferred from the claim text.