US 10,767,196 B2Granted

Engineering an increase in ethanol production by altering cofactor specificity

AssigneesEnchi Corporation, Dartmouth College, UT-Battelle, LLC

InventorsJonathan Lo, Adam M. Guss, Johannes P. Van Dijken, Arthur J. Shaw, IV, Daniel G. Olson, Christopher D. Herring, D. Aaron Argyros, Nicky Caiazza

Life of this patent

Estimating the term…

patentgrep

Loading patent…

Abstract

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Claims9

Dependent claims

A recombinant cellulolytic Clostridium host cell comprising at least one heterologous nucleic acid sequence encoding a bifunctional alcohol-acetaldehyde dehydrogenase, wherein the bifunctional alcohol-acetaldehyde dehydrogenase is AdhE;

wherein the bifunctional AdhE is encoded by a heterologous nucleic acid identical to a polynucleotide that encodes the polypeptide of SEQ ID NO:21, wherein the bifunctional AdhE uses NADPH as a cofactor instead of NADH;
wherein the recombinant Clostridium host cell further comprises a genetic modification that leads to the down-regulation of the native enzyme lactate dehydrogenase;
and wherein the recombinant Clostridium host cell has an increased production of ethanol compared to a wildtype cellulolytic Clostridium cell without the heterologous nucleic acid and the down-regulation of the native enzyme lactate dehydrogenase.

Dependencies are inferred from the claim text.

Facts

Application: 14/361,948Filed 30 Nov 2012 · us provisional application
Priority: 30 Nov 2011
Published: US 2014/0322783 A130 Oct 2014
Granted: 8 Sep 2020
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12P 7/06
Acyclic: ethanol, i.e. non-beverage
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 9/0008
Oxidoreductases: acting on the aldehyde or oxo group of donors
C12N 15/74
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…

Family

Application61/565,261Us provisional application · filed 30 Nov 2011
US 2014/0322783 A1Pre-grant publication · published 30 Oct 2014
US 10,767,196 B2This patent

From the US filing history. Foreign family members aren’t listed.

Timeline

Citations

Open elsewhere

Building an agent or pulling many patents? Use the API or MCP server instead of scraping pages. Get an API key