Brassicaceae plants resistant to Plasmodiophora brassicae (clubroot)

A method for producing a clubroot resistant Brassica plant comprising:

• introducing a CRL clubroot resistance locus in said Brassica plant, and selecting said CRL clubroot resistant Brassica plant for the presence of the CRL clubroot resistance locus by analyzing genomic DNA from said plant for the presence of at least one molecular marker, wherein said at least one molecular marker is linked to the CRL clubroot resistance locus, wherein said CRL clubroot resistance locus comprises the CRL1 and CRL2 clubroot resistance genes, wherein
• a) said CRL1 clubroot resistance gene comprises a nucleotide sequence
• i) having at least 95% sequence identity to the sequence of nucleotide positions 32750 to 51049 of SEQ ID NO:
• 1;
• ii) having a coding sequence having at least 95% sequence identity to the sequence of nucleotide positions 52 to 5343 of SEQ ID NO:
• 2, nucleotide positions 52 to 5340 of SEQ ID NO:
• 4, nucleotide positions 52 to 5361 of SEQ ID NO:
• 6, or to SEQ ID NO:
• 10;
• or
• iii) encoding a protein having at least 95% sequence identity to SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 7, or SEQ ID NO:
• 11;
• and
• b) said CRL2 clubroot resistance gene comprises a nucleotide sequence having at least 95% sequence identity to the sequence of nucleotide positions 220 to 2898 of SEQ ID NO:
• 8;
• or encoding a protein having at least 95% sequence identity to SEQ ID NO:
• 9;
• and
• generating progeny from the clubroot resistant Brassica plant comprising the CRL clubroot resistance locus, wherein said progeny is clubroot resistant and comprises said CRL clubroot resistance locus.

A Brassica plant or plant cell comprising a CRL1 and a CRL2 clubroot resistance gene as transgene, wherein

• a) said CRL1 clubroot resistance gene
• (i) comprises a coding sequence having at least 95% sequence identity to the sequence of nucleotide positions 52 to 5343 of SEQ ID NO:
• 2, nucleotide positions 52 to 5340 of SEQ ID NO:
• 4, nucleotide positions 52 to 5361 of SEQ ID NO:
• 6, or to SEQ ID NO:
• 10;
• or (ii) encodes a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 7, or SEQ ID NO:
• 11;
• and
• b) said CRL2 clubroot resistance gene comprises a coding sequence having at least 95% sequence identity to the sequence of nucleotide positions 220 to 2898 of SEQ ID NO:
• 8, or encodes a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 9.

A method for increasing clubroot resistance in a Brassica plant, comprising:

• a) providing a CRL1 and a CRL2 clubroot resistance gene as transgene, b) introducing the CRL1 and CLR2 gene into a Brassica plant cell, to create transgenic cells;
• and c) regenerating transgenic plants from said transgenic cells;
• wherein
• said CRL1 clubroot resistance gene i) comprises a coding sequence having at least 95% sequence identity to the sequence of nucleotide positions 52 to 5343 of SEQ ID NO:
• 2, nucleotide positions 52 to 5340 of SEQ ID NO:
• 4, nucleotide positions 52 to 5361 of SEQ ID NO:
• 6, or to SEQ ID NO:
• 10;
• or ii) encodes a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 7, or SEQ ID NO:
• 11;
• and wherein
• said CRL2 clubroot resistance gene comprises a nucleotide sequence having a coding sequence having at least 95% sequence identity to the sequence of nucleotide positions 220 to 2898 of SEQ ID NO:
• 8, or encodes a protein having at least 95% sequence identity to SEQ ID NO:
• 9.

A chimeric gene comprising the following operably linked elements:

• a) a heterologous plant-expressible promoter;
• b) a DNA sequence coding for a CRL1 or for a CRL2 protein;
• and
• c) optionally, a transcription termination and polyadenylation region functional in plant cells, wherein
• a) said DNA sequence coding for a CRL1 protein comprises a nucleotide sequence i) having at least 95% sequence identity to the sequence of nucleotide positions 52 to 5343 of SEQ ID NO:
• 2, nucleotide positions of 52 to 5340 of SEQ ID NO:
• 4, nucleotide positions of 52 to 5361 of SEQ ID NO:
• 6, or to SEQ ID NO:
• 10;
• or ii) encoding a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 7, or SEQ ID NO:
• 11;
• and
• b) said DNA sequence coding for a CRL2 protein comprises a nucleotide sequence having at least 95% sequence identity to the sequence of nucleotide positions 220 to 2898 of SEQ ID NO:
• 8;
• or encoding a protein having an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 9.