Identification and characterization of UDP-glucose:phloretin 4′-O-glucosyl transferase from Malus x domestica Borkh
AssigneeThe State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center)
InventorMwafaq Ibdah
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Abstract
A method of producing trilobatin is disclosed. The method comprising contacting a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 8 and having a 4′-O-glycosyltransferase activity with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, thereby producing trilobatin. A method of producing a plant which produces trilobatin, transgenic plant or plant cell and methods of producing transgenic plants are disclosed. Composition comprising trilobatin are also disclosed.
Claims7
A method of producing trilobatin, the method comprising contacting a polypeptide comprising an amino acid sequence at least 98% identical to SEQ ID NO:
- 8 and having a 4′-0-glycosyltransferase activity with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, thereby producing trilobatin, and purifying said trilobatin, wherein said polypeptide is exogenously expressed in a plant cell, a bacterial or a yeast cell.
A method of producing trilobatin, the method comprising transforming a plant cell, a bacterial cell or a yeast cell, with a polynucleotide encoding a polypeptide comprising an amino acid sequence at least 98% identical to SEQ ID NO:
- 8 and having a 4′-0-glycosyltransferase activity, thereby exogenously expressing said polypeptide in a plant cell, contacting said polypeptide with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, producing trilobatin in said plant cell, bacterial cell or yeast cell, and purifying said trilobatin.
Dependencies are inferred from the claim text.