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US 10,767,165 B2Granted
Reprogramming method for producing induced pluripotent stem cells (iPSC)
AssigneeCentre National de la Recherche Scientifique (CNRS)
InventorPierre Roux
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Abstract
The present invention is in the field of stem cells. Particularly, the present invention relates to a reprogramming method for producing induced pluripotent stem cells (iPSC) by transducing somatic differentiated cells with a viral vector expressing Δ133p53β isoform, Δ133p53γ isoform or both Δ133p53β and Δ133p53γ isoforms; to induced pluripotent stem cells as produced by this method; to uses thereof in cell therapy and as an in vitro model for studying various diseases; and to a method for detecting iPSC.
Claims13
An in vitro method for producing mammalian induced pluripotent stem cells (iPSCs), comprising:
- a) transducing isolated mammalian somatic cells with:
- i) at least one vector encoding a Δ133p53β isoform, Δ133p53γ isoform, or both Δ133p53β and Δ133p53γ isoforms, and
- ii) vectors encoding reprogramming factors Oct4, Sox2, Klf4, and cMyc;
- b) culturing the cells obtained in step a) in a medium supporting pluripotent cell expansion;
- and
- c) isolating iPSCs from the culture in step b).
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