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US 10,767,162 B2Granted
Methods for differentiation
AssigneePresident and Fellows of Harvard College
InventorsVictor Chun Li, Marc W. Kirschner
Life of this patent
Estimating the term…
Abstract
Described herein are methods relating to the differentiation of stem cells to more differentiated phenotypes, e.g. to terminally differentiated cell types and/or precursors thereof. In some embodiments, the methods relate to contacting the stem cells with differentiation factors and halting the cell cycle, thereby increasing the rate of differentiation.
Claims13
A method of differentiating a pluripotent stem cell, the method comprising:
- i) contacting the pluripotent stem cell with one or more nucleic acids encoding one or more terminal transcription factors;
- and
- ii) inhibiting the cell cycle of the pluripotent stem cell, wherein the cell cycle is inhibited by one or more of the following:
- reducing serum levels;
- reducing serum levels below 5%;
- contacting the cell with a PI3K inhibitor;
- contacting the cell with a Myc inhibitor;
- contacting the cell with a MAPK inhibitor;
- contacting the cell with a MEK1/2 inhibitor;
- contacting the cell with a CDK inhibitor;
- contacting the cell with an Id inhibitor;
- contacting the cell with a Ink family agonist;
- contacting the cell with a Cip/Kip family agonist;
- and culturing the cell in a media lacking a factor selected from the group consisting of:
- Bmp;
- Fgf;
- Activin;
- or TGFβ;
- wherein steps i) and ii) occur either:
- a) concurrently;
- or
- b) within 15 days of each other in either order;
- and
- whereby a differentiated cell is generated.
21 more elements
Dependencies are inferred from the claim text.