Methods for modulating production profiles of recombinant proteins
AssigneeAres Trading S.A.
InventorsAnais Muhr, David Bruhlmann, Martin Jordan, Herve Broly, Matthieu Stettler
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Abstract
The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer.
Claims3
A method of increasing production of a recombinant protein, said method comprising culturing a mammalian host cell expressing said protein in a cell culture medium comprising a triazine dye, wherein the triazine dye is selected from the group consisting of Cibacron Blue 3GA and Reactive Red 120 and wherein the triazine dye is present at a concentration of 0.01 μM to 150 μM, wherein production of the recombinant protein is thereby increased.
Dependencies are inferred from the claim text.