Devices, systems and methods for the production of humanized gut commensal microbiota

A method of ex vivo production of human commensal gut microbiota expressing a synthetic gene, the method comprising:

• screening a stool sample obtained from a healthy human subject comprising human commensal gut microbiota to detect and remove ova, parasites, and viruses;
• mixing the screened human commensal gut microbiota with a synthetic ex vivo culture medium to prepare a seed culture, wherein the culture medium comprises nutrients and metabolites present in the human intestine;
• infusing the seed culture into catridges coated with human cells, tissue extracts, adhesive proteins, or commensal colonizing factors;
• culturing the human commensal gut microbiota in the catridges, wherein the culturing comprises maintaining the temperature and gas gradients of the culture medium so as to be similar to the human gut, wherein the gas gradient comprises dissolved oxygen;
• modifying the cultured human commensal gut microbiota, wherein the modifying comprises infusing a synthetic gene into the culture medium in a concentration effective to be taken up by the cultured human commensal gut microbiota and
• harvesting the cultured human commensal gut microbiota.